22nd Annual Advanced Imaging Methods Workshop

January 21 – 23, 2025

aim2025.eventbrite.com

Registration by December 31 for Discounted Registration!

The Advanced Imaging Methods Workshop (AIM) is a 3-day event hosted by the CRL Molecular Imaging Center at UC Berkeley. AIM features new and emerging microscopy techniques and their applications in the biological sciences. We bring together internationally-renowned researchers in a broad variety of fields including biology, chemistry, physics, engineering, probe design, cellular biology, neuroscience, optics, metabolism and more. AIM has a particular focus on time-resolved techniques, such as time-correlated single photon counting and fluorescence lifetime imaging, as well as live cell and multiphoton microscopy.

In addition to invited talks from scientific leaders, AIM2025 will include demonstrations on-site and on campus, tours of UC Berkeley imaging facilities, lunch and coffee breaks, and evening receptions. There will also be a poster session and vendor fairs – both with prizes!

AIM includes lunch on-site each day, coffee breaks, and plenty of opportunities for interactions among participants. Registration is now open on Eventbrite: aim2025.eventbrite.com

Please reach out to us to Sponsor AIM 2025!

Preliminary Program

Tuesday, January 21

7:15 AM Registration and coffee

8:00 Welcome to AIM – Organizers

8:10 – 10:00 Fluorescence Lifetime Imaging

8:10 – 8:40 Wolfgang Becker TCSPC FLIM – Principles and Applications in Cell Metabolism

8:45 – 9:05 Marina Shirmanova FLIM of NAD(P)H for prognosis of tumor development and therapeutic response

9:10 – 9:30 Julia Martin Imaging Reversible Mitochondrial Membrane Potential Using an Enzymatically Uncaged Rhodamine Voltage Reporter

9:35 – 9:55 Jay Nadeau Evaluation of senolytic activity of encapsulated fisetin by fluorescence microscopy and FLIM

10:00 – 10:30 Break

10:30 – 12:05 Förster’s Resonant Energy Transfer Imaging

10:30 – 10:50 Ammasi Periasamy Investigation of mitochondrial metabolism in disease: Two-photon FLIRR Microscopy

10:55 – 11:15 Margarida Barroso Fluorescence Lifetime FRET to monitor target engagement in tumors at multiscale

11:20 – 11:25 Lucas Braun Quantitative FLIM Results in Zeiss Zen for the LSM980

11:25 – 11:45 Vendor Talks TBD

11:45 – 12:00 Adeela Syed BioImaging North America (BINA): Programs & Impact

12:05 Lunch

1:00 – 2:40 Single Molecule Imaging and Fluorescence Correlation Spectroscopy

1:00 – 1:20 Michael Börsch Beyond smFRET – NV qubit in nanodiamond for monitoring subunit rotation in single FoF1-ATP synthase

1:25 – 1:45 Sharonda LeBlanc Revealing biological pathways with time-resolved fluorescence

1:50 – 2:10 Brian Kobilka TBA

2:15 – 2:35 Michelle Digman Hyperspectral Phasor Analysis for Assessing Lipid Polarity in the Pathogenesis of Alzheimer’s Disease

2:40 – 3:00 Vendor Talks TBD

3:00 – 4:20 Vendor Fair

4:20 – 6:00 Innovations in Microscopy

4:20 – 4:40 Laura Waller Computational aberration correction

4:45 – 5:05 Cristina Rodríguez Into the deep: Next-generation optical microscopy for deep tissue imaging of the nervous system

5:10 – 5:30 Markita Landry Imaging Neuromodulation in the Brain with Near-Infrared Nanosensors

5:35 – 5:55 Aaron Streets TBA

6:00 – 8:00 Welcome Reception at Gather

Wednesday, January 22

8:00 Registration and coffee

8:30 – 10:00 Super Resolution Microscopy

8:30 – 9:10 Stefan Hell TBA

9:20 – 9:40 Ahmet Yildiz MINFLUX reveals dynamics of dynein stepping

9:45 – 10:05 Guang Gao Endoplasmic reticulum: nanodomain organization and contacts with mitochondria

10:10 – 10:15 Kristofer Fertig Abberior – Pushing the limits of Nanoscopy

10:15 Break

10:45 – 12:00 High Content 3D Imaging

10:45 – 11:05 Brandon Pekarek Imaging methods for interrogating gene editing in the CNS

11:10 – 11:30 Max Tauc-Adrian Cell morphology mirrors cellular function in high-content screens

11:30 – 11:50 Valentina Pedoia TBD

11:55 – 12:15 Sebastian Ortiz and Jason Fung High Content 3D Imaging and Segmentation with the ZEISS Celldiscoverer 7 and Arivis Pro

12:15 Lunch

1:00 – 3:30 Artificial Intelligence and Deep Learning in Microscopy

1:00 – 1:20 Gokul Upadhyayula TBD

1:25 – 1:45 Viren Jain Mapping the Brain

1:50 – 2:10 Loic A. Royer Machine Intelligence for Microscopy: From Tracking to Omega

2:15 – 2:35 Bo Huang Mapping the inner world of cells

2:40 – 3:00 Jan Funke What is all that AI good for anyway?

3:05 – 3:20 Leica TBA

3:30 – 6:00 Demonstrations & Vendor Fair

Please note – some demonstrations are a 10 – 15 minute walk, see map.

6:00 – 8:00 Poster Session and Reception

2nd Floor Lobby, Li Ka Shing Building

Bring your badge for access! We will have security on site!

Thursday, January 23

7:30 Registration and coffee

8:30 – 10:20 New Techniques

8:30 – 8:50 Na Ji Machine-learning-based adaptive optics for in vivo fluorescence microscopy

8:55 – 9:15 Jennifer R. Cochran TBD

9:20 – 9:50 Luke Lavis Building Brighter Fluorophores for Advanced Imaging

9:55 – 10:15 Shalin Mehta Self-supervised modeling of cellular responses to perturbations

10:20 Break

10:50 – 12:30 Neuroscience

10:50 – 11:10 Diana Bautista Pain goes viral: Neural mechanisms of coronavirus pain and inflammation

11:15 – 11:35 Elizabeth Hillman TBA

11:40 – 12:00 Hillel Adesnik All optical electrophysiology

12:05 – 12:25 Stephen Brohawn TBA

12:30 Lunch

1:15 – 2:30 Vendor Fair

2:30 – 4:00 Probe Development

2:30 – 2:50 Colleen Scott Xanthene-based NIR/SWIR dyes for bioimaging

2:55 – 3:15 Evan Miller Let there be light! Building molecules to visualize cell physiology

3:20 – 3:50 Natalie Fardian-Melamad TBD

4:00 Break

4:30 – 6:15 Metabolic Imaging

4:30 – 4:50 Shagufta Alam From Fundamental to Translational Research: Understanding Mitochondrial Bioenergetics in Prostate Cancer by Multiphoton FLIM

4:55 – 5:15 Melissa Skala NAD(P)H FLIM reveals immediate metabolic shifts of neutrophils with activation across biological systems

5:20 – 5:50 Angelika Rück TBA

5:55 – 6:10 Wolfgang Becker Advances in FLIM Data Analysis

6:15 – 8:00 Farewell Reception on site

Organizers

Holly Aaron, Molecular Imaging Center, University of California, Berkeley
Luis Alvarez, Molecular Imaging Center, University of California, Berkeley
Wolfgang Becker, Becker-Hickl, Germany
Feather Ives, Molecular Imaging Center, University of California, Berkeley
Adeela Syed, Optical Biology Facility, University of California, Irvine
Gokul Upadhyayula, Advanced BioImaging Center, University of California, Berkeley

Register here: