Instrumentation & Fees

• Instruments in:
• 491 LSA
• 461 LKS

The CRL Flow Cytometry Facility operates in two locations: 491 Life Science Addition (LSA) and 461 Li Ka Shing (LKS) Building. There are 2 sorters and 4 analyzers in LSA; and there is 1 sorter and 1 analyzer in LKS.

Fees

Training fees for both Cell Analyzers and Cell Sorters:

• $150/session (UC Berkeley internal rate)
• $300/session (external rate)

User self-operated cell sorting (3 training sessions required; highly recommended for increased user scheduling flexibility and instrument availability) rates are as follows:

• $73/h (UC Berkeley internal rate)
• $146/h (external rate)

Analysis is performed by users (1 training session required). Rates for analyzer use are as follows:

• $45/h (UC Berkeley internal rate)
• $90/h (external rate)

Staff-assisted cell sorting (limited to staff availability; limited to 1-2 sorts per month) rates are as follows :

• $95/h (UC Berkeley internal rate)
• $190/h (external rate)

Signing up for sorting or analysis or training

Reservations are handled by an online system called iLab. Users must obtain an iLab account before signing up to use the sorters or analyzers.

Once you have an iLab account, you can log in to iLab in order to request time for sorting or training. Requests for sorting or training must be approved by staff.
Once you are trained you can use iLab to sign up to use the analyzers.

Instruments in 491 LSA

The flow core located in room 491 Life Sciences Addition building (LSA) is managed by Hector Nolla. This core houses two cell sorters: a BD Influx (LSA) and a Beckman-Coulter Moflo. In addition, the LSA facility houses four analyzers: BD LSR II, BD LSR Fortessa, BD LSR Fortessa X20, and a Beckman-Coulter FC500.

Alma Valeros and Hector Holla operate the cell sorters. They also assist users with the analyzers.

Hours of operation are from 9:30AM -8:30PM, Monday to Fridays.
These are the only hours during which sorting can be performed. Trained users can access the analyzers 7 days a week, 24 hours a day.

You can call the facility: (510) 642-2843.

Sorters in LSA

Influx Cell Sorter (491 LSA)

Aria Fusion (2016) (491 LSA);

Analyzers in LSA

LSR Fortessa X20 Analyzer (2013) (491 LSA)

LSR Fortessa Analyzer (2010) (491 LSA)

LSR II Analyzer (2007) (491 LSA)

Instruments in 461 LKS

The Li Ka Shing (LKS) flow core is located in room 461. This core is managed by Kartoosh Heydari. It houses a BD Influx cell sorter and a BD LSR Fortessa analyzer.

Kartoosh operates the cell sorter and assists users with the analyzer. This core is part of the CIRM Institute at UC Berkeley.

Hours of operation are from 9:30AM -5:00PM, Monday to Fridays.
These are the only hours during which sorting can be performed in this facility. The analyzers are available for use 24h a day, 7 days a week.

You can contact the facility by calling (510) 664-4308 (Lab) or (510) 664-4307 (Office)

Sorter in LKS

Influx Cell Sorter (2013) (LKS 461)

LSR Fortessa Analyzer (2010) (491 LSA)

Read More

• Flow Cytometry Facility
• FCF Facility News
• Online scheduling and access to the facility
• Instrumentation & Fees
• FAQ & Training
• Staff and Contact