Education – Microscopy Background

Need to brush up on microscopy basics? There is so much out there on the web. Here are a few great places to start:

Microscopy Primers

• Florida State’s Optical Microscopy Primer
• Microscopy Education’s Primer

Courses

For-credit courses at UC Berkeley:

• PMB 185: Techniques in Light Microscopy
• MCB C205 / PHY C205: Modern Optical Microscopy
• BioE 168L: Practical Light Microscopy
• EE 290-003: Computational Imaging

Workshops at UC Berkeley:

• Advanced Imaging Methods Workshop, UC Berkeley
• Plant & Animal Microtechnique

Courses and Workshops at Other Institutions:

• FRET Workshop, UVA Keck Center
• Optical Microscopy & Imaging in the Biomedical Sciences, Woods Hole
• Analytical & Quantitative Light Microscopy, Woods Hole
• Quantitative Imaging: From Cells to Molecules, Cold Spring Harbor Lab
• Quantitative Fluorescence Microscopy, Mount Desert Island
• European Light Microscopy Initiative

Microscopy Videos on YouTube

You can search for a variety of microscopy topics on YouTube. Here are a few links to Harvard Medical School Imaging Facility videos by Jennifer Waters:

• Numerical Aperture
• Signal-to-Noise Ratio
• Point Spread Function
• Preventing Objective Lens Damage: Blunt Force Trauma
• Preventing Objective Lens Damage: Immersion Oil Problems
• Fluorescent Filters: Choosing a Filter Set
• Fluorescent Filters: Multiple Fluorophores & Bleedthrough

Free Image Analysis Software

• Fiji
• CellProfiler
• Advice for Learning Fiji or ImageJ
• Zen Lite (PC only)
• Nikon Elements Viewer

Spectral & Fluorophore Databases

• Semrock Searchlight
• Fluorescent Proteins
• Ulbrich Online Spectra Viewer
• Life Technologies SpectraViewer
• BD Biosciences
• Chroma Spectra Viewer

Bay Area Imaging Facilities

• CIRM/QB3 Shared Stem Cell Facility , UCB (Stanley Hall, contact Mary West)
• The Biological Imaging Facility, UCB (Koshland Hall, contact Denise Schichnes)
• Advanced Bioimaging Center, UCB (Barker Hall, contact Gokul Upadhyayula)
• Electron Microscopy Facility, UCB (Barker Hall, contact Danielle Jorgens)
• Nikon Imaging Center, UCSF (Genentech Hall, contact DeLaine Larsen)

Manufacturers

• 3i
• arivis
• Becker-Hickl
• Bitplane (Imaris)
• Boston Electronics
• Coherent
• Imagine Optics
• Leica
• Olympus
• Nikon
• Spectra-Physics
• Sutter
• Warner
• Zeiss
  • Zeiss Slide Scanning References

List Servers/Community

• Confocal Listserv
• BioImaging North America (BINA)
• ABRF – Association of Biomedical Research Facilities

Fun

• Sugru
• Arc of Reflection