Draft Agenda
| Organizers | Speakers | Sponsors | Agenda | Registration |
| Conduct | Location | Posters | Demos | Abstracts |
| Day 1 | Day 2 | Day 3 | Abstracts | Breakout Sessions |
PDF of Full Agenda Here
Wednesday, January 29
Registration & Coffee 7:00 AM
Welcome 8:00 – 8:40 AM
Holly Aaron (University of California, Berkeley), Opening Remarks
Wolfgang Becker (Becker-Hickl, GmbH), Introduction to time-correlated fluorescence lifetime imaging (TCSPC FLIM)
Short Break 8:40 – 9:00 AM
Fluorescent Lifetime Imaging I (Chair: Wolfgang Becker) 9:00 – 10:15 AM
Horst Wallrabe (University of Virginia), Autofluorescence – multiexponential or incomplete…..that’s the question
Vladislav Shcheslavskiy (Becker-Hickl, GmbH), Oxygen measurements by time-correlated single photon counting
Angelika Rück (University of Ulm), NADH/FAD/FMN-FLIM in bioenergetic alterations
VENDOR FAIR I 10:15 – 11:15 AM
Metabolic investigations in clinical imaging (Chair: Hauke Studier) 11:15 AM – 12:30 PM
Ammasi Periasamy (Keck Center, University of Virginia), Investigation of metabolism in living prostate cancer cells: FLIM-FLIRR microscopy
Melissa Skala (University of Wisconsin, Madison), Metabolic imaging of immune cell function
Michael Roberts (University of Queensland Diamantina Institute), Using FLIM to characterise redox changes in liver injury and disease
LUNCH 12:30 – 2:00 PM
Neuroscience (Chair: Andrea Gomez, University of California, Berkeley) 2:00 – 3:40 PM
Evan Miller (University of California, Berkeley), Electrophysiology, unplugged: Fluorophores to measure voltage in living systems
Fangyuan Ding (California Institute of Technology), Constitutive splicing and economies of scale in gene expression
Saskia de Vries (Allen Brain Institute), A large-scale standardized calcium imaging pipeline reveals functional organization of the mouse visual cortex
Na Ji (University of California, Berkeley), Imaging neural activity at high spatiotemporal resolution
VENDOR FAIR II 3:45 – 5:00 PM
Or BREAKOUT SESSION 4:00 – 4:45PM (Venetian Room)
Ariel Waldman (Global Director of Science Hack Day), Life under the ice
Innovations in Imaging I (Chair: Benjamin Smith, University of California, Berkeley) 5:00 – 6:15 PM
Meredith Calvert (Denali Therapeutics), Advanced imaging approaches in drug discovery: Exploring macromolecular biodistribution to brain targets
Aaron Streets (University of California, Berkeley), Imaging and sequencing single cells
Laura Waller (University of California, Berkeley), 3D phase microscopy with scattering samples
[7PM – Dinner on your own or Speaker Dinner for Speakers, Organizers and Chairs]
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Thursday, January 30
Coffee 7:30 AM
Developing Microscopy Tools for the Future (Chair: DeLaine Larsen, University of California, San Francisco) 8:00 – 9:40 AM
Mateo Sanchez-Lopez (Stanford University), Directed evolution of TEV protease and application to fluorescent reporters of Ca+2 activity and GPCR activity in cells
Kwanghun Chung (Massachusetts Institute of Technology), Towards holistic imaging and rapid phenotyping of complex biological systems
Xiaoyu Shi (University of California, Irvine), Label-retention expansion microcopy enables molecular-resolution imaging
Joshua Vaughan (University of Washington), Super-resolution microscopy made simple
Break 9:40 – 10:00 AM
Fluorescence Lifetime Imaging II (Chair: Denise Schichnes, University of California, Berkeley) 10:00 – 10:50 AM
Emilie Steinmark (Kings College London), Combining fluorescent molecular rotors and time-resolved fluorescent anisotropy in viscosity imaging
Alzbeta Chorvatova (International Laser Centre, Bratislava, Slovakia), Environmental sensing by time-resolved autofluorescence monitoring in algae
Short break 10:50 – 11:00 AM
Innovations II (Chair: Blaise Ndjamen, Gladstone Institutes) 11:00 AM – 12:30 PM
Samantha Fore (Zeiss Microscopy), Multiplex mode for the LSM 9 series with Airyscan 2: Fast and gentle confocal super-resolution in large volumes
Alberto Diaspro (Nanoscopy, Istituto Italiano di Tecnologia, Genova, Italy), Multi-messenger optical microscopy coupling fluorescence and label-free imaging
Elizabeth Hillman (Columbia University), New developments and applications of SCAPE microscopy for fast 3D imaging
Sara Abrahamsson (University of California, Santa Cruz), M25 multifocus 25-plane microscope with camera array for neural imaging
LUNCH 12:15 – 1:30 PM
Investigating Mitochondrial Action (Chair: Samantha Lewis, University of California, Berkeley) 1:45 – 3:00 PM
John Albeck (University of California, Davis), Live-cell analysis of ATP homeostasis in single cells
Laura Newman (Salk Institute), Mitochondrial/ER contacts modulate release of mtDNA from mitochondria
Natalie Porat-Shliom (National Cancer Institute, National Institutes of Health), Mitochondrial dynamics in intact tissues using intravital microscopy
VENDOR FAIR III 3:00 – 4:45 PM
Or BREAKOUT SESSION 3:15 – 4:30 PM (Venetian Room)
Gender-inclusion in microscopy
(Chairs: Meredith Calvert and Gisele Giorgi, BioImaging North America Diversity Working Group, Abby Dernburg, UC Berkeley)
Computation & Analysis I (Chair: Deepa Sridharan) 4:45 – 6:00 PM
Kevin Eliceiri (University of Wisconsin, Madison), The ImageJ ecosystem: An open and extensible platform for biomedical image analysis
Beth Cimini (Broad Institute), Getting the most data from your images with CellProfiler and morphological profiling
Loic Royer (CZ Biohub), noise2self and napari — novel tools to enhance and visualize microscopy data
POSTER SESSION & RECEPTION 6:00 – 8:30 PM (Venetian Room)
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Friday, January 31
Coffee 8:00 AM
Microscopy in a Clinical Setting (Chair: Annette Chan, San Francisco State University) 8:30 – 9:55 AM
Wolfgang Becker (Becker-Hickl), Cancer detection by NADH/FAD FLIM
Yousuf Mohammed (University of Queensland Diamantina Institute), Imaging topical drug pharmacokinetics in human skin – Opportunities and challenges
Marina Shirmanova (Privolzhsky Research Medical University, Russia), What FLIM of NAD(P)H reveals about tumor metabolism
ON- AND OFF-SITE DEMOS 10:15 AM – 12:15 PM
TUTORIALS 10:15 – 12:30
10:15 – 11:00 AM Fluorescence Lifetime Imaging Analysis – Wolfgang Becker
11:00 – 11:45 AM FIJI is Just ImageJ – Kevin Eliceiri
11:45 AM – 12:30 PM Introduction to CellProfiler – Beth Cimini
LUNCH 12:30 – 1:30 PM
POSTER WINNER Announcement 2:00 – 2:10 PM
Industry Innovations (Chair: Eva Nichols) 2:10 – 2:45 PM
Steven Smith (Refined Optical Systems), TBA
Jim Sims (Hamamatsu), TBA
TBA (ThorLabs), Bessel beam
Byron Hartman (Miltenyi BioTec), Exploring the architecture of biology with tissue clearing and light sheet microscopy
Short Break 2:45 – 3:00
Subcellular Imaging (Chair: Julia Lazzari-Dean, University of California, San Francisco) 3:00 – 4:15 PM
Thomas Gensch (Cellular Biophysics, Forschungszentrum Jülich), Fluorescence lifetime imaging of intracellular sodium concentration
Abby Dernburg (University of California, Berkeley), Measuring diffusion within a subcellular compartment
Steven Vogel (NIAAA/NIH), Using FRET spectroscopy and FCS to study conformational changes associated with Venus-tagged CaMKII activation and T-site interactions
Dessert break 4:15 – 4:45 PM
Computation (Advanced Bioimaging Center Edition, Chair: Gokul Upadhyayula & Eric Betzig) 4:45 – 6:30 PM
Yair Rivenson ((University of California, Los Angeles), Deep learning enhanced fluorescence microscopic imaging
Aydogan Ozcan (University of California, Los Angeles), Deep learning-enabled computational microscopy and sensing
Nicholas Sofroniew (Chan Zuckerberg Initative), napari: A multi-dimensional image viewer for python
Kristen Kwan (University of Utah), Computational approaches to dissecting developmental tissue morphogenesis
CLOSING RECEPTION (6:30 – 8:00PM)
Start times each day will be 8:00AM and most talks will end by ~6:30pm. Receptions and poster sessions will begin at the end of talks.
